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C-Kit/CD117 Rabbit Polyclonal Antibody (PE)

SKU: orb499229

Description

C-Kit/CD117 Rabbit Polyclonal Antibody (PE)

Images & Validation

Tested ApplicationsFC, IF
Dilution rangeFlow-Cyt=1μg /test, IF=1:100-500
ReactivityHuman, Mouse
Predicted ReactivityEquine

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human c-Kit/CD117 (451-550/976aa)
TargetKIT
PurificationAffinity purified by Protein A
ConjugationPE

Storage & Handling

StorageShipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
DisclaimerFor research use only

Alternative Names

C Kit; c-Kit; CD 117; CD117; CD117 antigen; KIT; KIT_HUMAN; Mast/stem cell growth factor receptor; Mast/stem cell growth factor receptor Kit; p145 c-kit; PBT; Piebald trait protein; Proto oncogene c Kit; Proto oncogene tyrosine protein kinase Kit; Proto-oncogene c-Kit; SCF Receptor; SCFR; soluble KIT variant 1; Stem cell factor receptor; tyrosine protein kinase Kit; Tyrosine-protein kinase Kit; v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog; v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog.

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

C-Kit/CD117 Rabbit Polyclonal Antibody (PE)

Blank control: HUVEC. Primary Antibody (green line): Rabbit Anti-c-Kit antibody (orb499229), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

C-Kit/CD117 Rabbit Polyclonal Antibody (PE)

Blank control: K562. Primary Antibody (green line): Rabbit Anti-c-Kit antibody (orb499229), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

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Protocol Information

FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol

C-Kit/CD117 Rabbit Polyclonal Antibody (PE) (orb499229)

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Available Sizes

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100 μl
¥ 7,020.00
DispatchUsually dispatched within 2-3 weeks
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