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Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 8
| Tested Applications | WB |
|---|---|
| Dilution Range | WB=1:2000-5000 |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone No. | 4G12 |
| Immunogen | Recombinant human Caspase 8 Protein |
| Target | CASP8 |
| Molecular Weight | 43 kDa |
| Purification | Affinity purified by Protein G |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ALPS2B; CAP4; Casp-8; FLICE; MACH; MCH5; CD20; CK-20; CK20; K20; KRT21; CASP8_HUMAN; CASP8; Apoptotic cysteine protease; Apoptotic protease Mch-5; FADD-homologous ICE/ced-3-like protease; FADD-like ICE (FLICE); ICE-like apoptotic protease 5; MORT1-associated ced-3 homolog (MACH); 3.4.22.61; CASP8_MOUSE; CASP8_RAT;
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Paraformaldehyde-fixed, paraffin embedded (Mouse liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Caspase 8) Monoclonal Antibody, Unconjugated (orb500937) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat lymphoid), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Caspase 8) Monoclonal Antibody, Unconjugated (ascites of orb500937 4G12) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat lymphoid), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Caspase 8) Monoclonal Antibody, Unconjugated (ascites of orb500937 4G12) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat lymphoid), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Caspase 8) Monoclonal Antibody, Unconjugated (ascites of orb500937 4G12) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat lymphoid), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Caspase 8) Monoclonal Antibody, Unconjugated (ascites of orb500937 4G12) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
Sample: 293T-UV (Human) Cell Lysate at 30 ug, U251-UV (Human) Cell Lysate at 30 ug, Primary: Anti-Caspase 8 (orb500937) at 1/4000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 41'43'55 kD, Observed band size: 43 kD.
Sample: Hela (Human) Cell Lysate at 30 ug Jurkat (Human) Cell Lysate at 30 ug Molt-4 (Human) Cell Lysate at 30 ug K562 (Human) Cell Lysate at 30 ug Primary: Anti-Caspase 8 (orb500937) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41'43'55 kD Observed band size: 43 kD.
Sample: Stomach (Mouse) Lysate at 40 ug, Spleen (Mouse) Lysate at 40 ug, Lymph node (Mouse) Lysate at 40 ug, Urinary bladder (Mouse) Lysate at 40 ug, Primary: Anti-Caspase 8 (orb500937) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 41'43'55 kD, Observed band size: 55'53D.
Quick Database Links
Gene Symbol
CASP8
UniProt
UniProt Details
− No UniProt data available
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Caspase 8 Mouse Monoclonal Antibody (orb500937)
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