Caspase-8 subunit p10 Rabbit Polyclonal Antibody

Blank control: K562 (fixed with 80% methanol (5 min) and and then permeabilized with 0.01M PBS-Tween for 20 min). Primary Antibody: Rabbit Anti-caspase-8 antibody (orb500973, Green), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Paraformaldehyde-fixed, paraffin embedded (rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Caspase 8) Polyclonal Antibody, Unconjugated (orb500973) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Raw264.7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Caspase-8 subunit p10) polyclonal Antibody, Unconjugated (orb500973) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Thymus (Mouse) Lysate at 40 ug, Primary: Anti-Caspase 8 (orb500973) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 12, 55 kD, Observed band size: 17 kD.