CCR5 Rabbit Polyclonal Antibody

SKU: orb13298

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Description

Images & Validation

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Item 1 of 2

Tested Applications	WB
Dilution range	WB=1:500-2000
Reactivity	Human
Predicted Reactivity	Mouse, Rat

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human CCR-5 (201-300/352aa)
Target	CCR5
Molecular Weight	40 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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C-C chemokine receptor 5; CCR5; CC-CKR-5; CCR-5; CCCKR5; CD195; CD 195; CKR-5; CKR5; CMKBR5; FLJ78003; IDDM22; AM4-7; C C chemokine receptor type 5; C-C motif chemokine receptor 5 A159A; CC Chemokine Receptor Type 5; CCR5 chemokine(C C motif) receptor 5; Chemokine receptor CCR5; CHEMR13; HIV 1 Fusion Coreceptor; HIV1 fusion coreceptor; IDDM22; MIP-1 alpha receptor; CCR5_HUMAN.

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Blank control (blue): U937 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-CCR5 antibody (orb13298), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1X PBS containing 0.5% BSA.

Sample: Lane 1: Human THP-1 cell lysates, Lane 2: Human U937 cell lysates, Primary: Anti-CCR-5 (orb13298) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 40 kD, Observed band size: 43 kD.

Quick Database Links

Gene Symbol

CCR5

UniProt

P51681

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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