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CD11b Rabbit Polyclonal Antibody
SKU: orb11009
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Description
Images & Validation
−Item 1 of 2
| Tested Applications | WB |
|---|---|
| Dilution range | WB=1:500-2000 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Porcine |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from mouse CD11b (77-180/1152aa) |
| Target | ITGAM |
| Molecular Weight | 165-170 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−ITAM_HUMAN; Integrin alpha-M; CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Leukocyte adhesion receptor MO1; Neutrophil adherence receptor; CD11b; ITGAM; CD11B; CR3A;
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Blank control: U937 (blue). Primary Antibody: Rabbit Anti-CD11b antibody (orb11009), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (orb11009, 1 µg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.
Sample: Lane 1: Mouse Lymph node tissue lysates, Lane 2: Mouse Spleen tissue lysates, Lane 3: Rat Spleen tissue lysates, Primary: Anti-CD11b (orb11009) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 125 kDa, Observed band size: 175 kDa.
Quick Database Links
Gene Symbol
ITGAM
UniProt
UniProt Details
− No UniProt data available
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CD11b Rabbit Polyclonal Antibody (orb11009)
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