Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human Fc gamma RII a (201-300/317aa)
Target	FCGR2A
Molecular Weight	40 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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CD 32; CD32A; CD32B; CD32C; CDw32; Fc gamma RII; low affinity II receptor; Fc gamma receptor II; Fc-GAMMA-RII; Fc[g]RII; IgG Fc receptor II; FCG 2; FCG2; FcgammaRII; FCGR 2; FcGR; FCGR2; FCGR2A; FCGR2A1; FCGR2B; FCGR2C; FcgRII; Fcr-2; Fcr-3; FcRII a; FCRII; FcRII b; FcRII c; FcRIIC; IGFR 2; IGFR2; Ly-17; Ly-m20; LyM-1; Lymphocyte antigen 17; MGC23887; MGC30032; FCG2C_HUMAN.

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Blank control: U937 (blue). Primary Antibody: Rabbit Anti-CD32 antibody (orb13406), Dilution: 0.2 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (orb13406, 0.2 µg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.

Sample: Jurkat (Human) Cell Lysate at 30 ug, Primary: Anti-CD32 (orb13406) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 31 kD, Observed band size: 31 kD.

Sample: K562 Cell Lysate at 30 ug, Primary: Anti-CD32 (orb13406) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 31 kD, Observed band size: 33 kD.

Quick Database Links

Gene Symbol

FCGR2A

UniProt

P12318

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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