Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human CD53 (65-160/219aa)
Target	CD53
Molecular Weight	24 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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AI323659; Antigen MOX44 identified by monoclonal; MRC-OX44; CD 53; CD53 antigen; CD53 antigen, isoform CRA_b; CD53 glycoprotein; Cd53 molecule; CD53 tetraspan antigen; CD53_HUMAN; Cell surface glycoprotein CD53; Leukocyte antigen MRC OX-44; Leukocyte surface antigen CD53; MOX 44; MOX44; Ox-44; OX44; RATOX44; Tetraspanin 25; Tetraspanin-25; Tetraspanin25; Transmembrane glycoprotein; TSPAN 25; Tspan-25; TSPAN25.

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Sample: Jurkat (Human) Cell Lysate at 30 ug, Molt-4 (Human) Cell Lysate at 30 ug, Primary: Anti-CD53 (orb156312) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 24 kD, Observed band size: 24 kD.

Sample: MOLT-4 (Human) Cell Lysate at 30 ug, Primary: Anti-CD53 (orb156312) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 24 kD, Observed band size: 25 kD.

Sample: Molt-4 (Human) Cell Lysate at 30 ug, Primary: Anti-CD53 (orb156312) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 24 kD, Observed band size: 26 kD.

Tissue/Cell: human colon cancer, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CD53 Polyclonal Antibody, Unconjugated (orb156312) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

CD53

UniProt

P19397

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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