Claudin 5 Rabbit Polyclonal Antibody

SKU: orb182867

Featured

Description

Research Area

Signal Transduction

Images & Validation

−

Item 1 of 3

Tested Applications	FC, IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Equine, Porcine, Rabbit, Rat, Sheep
Application Notes	A customer has reported negative results in live-cell applications for FACS, indicating that a fixation step may be required.

Related Conjugates & Formulations

−

Key Properties

−

Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human Claudin 5 (29-81/218aa)
Target	CLDN5
Molecular Weight	23 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

−

AWAL; BEC 1; BEC1; Claudin 5(transmembrane protein deleted in velocardiofacial syndrome); Claudin5; Claudin-5; CLDN 5; CLDN5; CPETR L1; CPETRL 1; CPETRL1; TMDVCF; TMVCF; Transmembrane protein deleted in VCFS; Transmembrane protein deleted in velocardiofacial syndrome; Androgen withdrawal and apoptosis induced protein RVP1 like; CLD5_HUMAN.

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Protein: lung (mouse) lysates at 45 ug, Primary: Anti-Claudin 5 (orb182867) at 1:300, Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1:5000, ECL excitated the fluorescence, Predicted band size: 23 kD, Observed band size: 23kD.

Blank control: K562. Primary Antibody (green line): Rabbit Anti-Claudin 5 antibody (orb182867), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 0.5 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Claudin 5) Polyclonal Antibody, Unconjugated (orb182867) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

CLDN5

UniProt

O00501

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC-P

Immunohistochemistry Paraffin

View Protocol

IHC-Fr

Immunohistochemistry Frozen

Flow Cytometry

Immunofluorescence

Filter by Applications

IF (1)
WB (2)

Filter by Species

Mouse (3)

Maysam Havasi Mehr 1, Shahein Momenabadi 2, Ali Vakili 3, Abbas Pakdel 4, Abbas Ali Vafaei 5, Abedin Vakili Neuroprotective effects of Daphnetin on hippocampal neurons and blood-brain barrier integrity in a mouse model of cerebral ischemia Brain Res Bull ., (2024)

PubMed: 39447767

Applications

Reactivity

Mouse

Yuanyuan Bai 1 2, Haiyan Wu 1 3, Xu Wang 4, Yang Guo 1 2, Bingqing Gong 1 2, Beibei Dong 2, Yonghao Yu A mitochondrial-derived peptide MOTS-c contributes to the protective effect against brain injury associated with LPS-induced sepsis by strengthening the blood-brain barrier's ultrastructure Int J Neurosci, (2025)

PubMed: 40753494

Applications

Reactivity

Mouse

Jessica J Avilez-Avilez 1 2, Jesús Enrique García-Aviles # 1 3, Ricardo Jair Ramírez-Carreto # 4 5, Verónica Salas-Venegas 4 6, Mara A Guzmán-Ruiz 3, Fernanda Medina-Flores 7, Mina Königsberg 8, Anahí Chavarría 9, Beatriz Gómez-González Progressive Blood-Brain Barrier Disruption in Sleep-Restricted Young Mice: Cellular Senescence and Neuroinflammation Crosstalk Neurochem Res ., (2025)

PubMed: 40824389

Applications

Reactivity

Mouse

Maydelín Espadín a b , Miguel Cervantes c , Angélica Coyoy-Salgado d , Claudia Espinosa-Garcia e , Beatriz Gómez-González b , Gabriela Moralí Progesterone restores blood-brain barrier and memory after chronic cerebral hypoperfusion in male rats Brain Behavior and Immunity Integrative, (2025)

PubMed: