Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from mouse Claudin 1 (121-211/211aa)
Target	CLDN1
Molecular Weight	23 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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CLD1_HUMAN; Claudin-1; Claudin 1; Claudin1; CLD1; SEMP1; Senescence-associated epithelial membrane protein; UNQ481/PRO944; ILVASC;

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Sample: Lane 1: Mouse Skin tissue lysates, Lane 2: Human A549 cell lysates, Primary: Anti-CLDN1 (orb10445) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 23 kDa, Observed band size: 17 kDa.

Tissue/Cell: rat intestine tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Claudin-1 Polyclonal Antibody, Unconjugated (orb10445) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

CLDN1

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Protocol Information

Western Blot (IB, immunoblot)

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Mouse (1)

Ningbo Qin 1, Hongxu Liu 2, Xinru Wang 2, Yi Liu 2, Hong Chang 2, Xiaodong Xia Sargassum fusiforme polysaccharides protect mice against Citrobacter rodentium infection via intestinal microbiota-driven microRNA-92a-3p-induced Muc2 production Int J Biol Macromol, (2025)

PubMed: 39863236

Applications

Reactivity

Mouse