CLEC7A Rabbit Polyclonal Antibody

SKU: orb13296

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Description

Research Area

Inflammation, Macrophage

Images & Validation

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Item 1 of 5

Tested Applications	FC, WB
Dilution Range	WB=1:500-2000, Flow-Cyt=1ug/Test
Reactivity	Human, Mouse
Predicted Reactivity	Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human CLEC7A (41-140/247aa)
Target	CLEC7A
Molecular Weight	28 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CLC7A_HUMAN; C-type lectin domain family 7 member A; BGR; beta GR; CLECSF12; DECTIN1; beta-glucan receptor; beta Glucan Receptor; C-type lectin superfamily member 12; C type calcium dependent carbohydrate recognition domain lectin superfamily member 12; Dendritic cell-associated C-type lectin 1; DC-associated C-type lectin 1; Dectin-1; Dectin 1; Dectin1; CD369; UNQ539/PRO1082;

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Blank control: RAW264.7. Primary Antibody (green line): Rabbit Anti-Iba1 antibody (orb13296), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-Rabbit IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-beta Glucan Receptor antibody (orb13296), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Mouse Spleen tissue lysates, Lane 2: Mouse Lung tissue lysates, Primary: Anti-beta Glucan Receptor (orb13296) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 28 kDa, Observed band size: 28, 47 kDa.

Sample: Lung (Mouse) lysate at 30 ug, Primary: Anti-beta Glucan Receptor (orb13296) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000 dilution, Predicted band size: 28 kD, Observed band size: 28 kD.

Sample: Spleen (Mouse) Lysate at 40 ug, Thymus (Mouse) Lysate at 40 ug, Primary: Anti-beta Glucan Receptor (orb13296) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 28 kD, Observed band size: 28 kD.

Quick Database Links

Gene Symbol

CLEC7A

UniProt

Q9BXN2

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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Find More Protocols

Pagliari, C. et al. Paracoccidioidomycosis: characterization of subpopulations of macrophages and cytokines in human mucosal lesions Med Mycol, 57, 757-763 (2019)

PubMed: 30418569