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Collagen II Rabbit Polyclonal Antibody
Description
Images & Validation
−| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Reactivity | Human, Mouse, Rabbit |
| Predicted Reactivity | Bovine, Canine, Gallus, Guinea pig, Porcine, Rat |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human Collagen II (1201-1300/1487aa) |
| Target | COL2A1 |
| Molecular Weight | 117 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
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Sample: 293T (human) cell Lysate at 40 ug, A549 (human) cell Lysate at 40 ug, MCF-7 (human) cell Lysate at 40 ug, HepG2 (human) cell Lysate at 40 ug, Placenta (mouse) Lysate at 40 ug, MG63 (human) cell Lysate at 40 ug, Primary: Anti-Collagen II (orb221349) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 117 kD, Observed band size: 120 kD.
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UniProt Details
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Protocol Information
Fu, Na et al. PCL-PEG-PCL film promotes cartilage regeneration in vivo Cell Prolif, 49, 729-739 (2016)
Collagen II Rabbit Polyclonal Antibody (orb221349)
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