CSF1 Rabbit Polyclonal Antibody

SKU: orb325141

Description

Rabbit polyclonal antibody to CSF1

Research Area

Cell Biology, Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 10

Tested Applications	ICC, IF, IHC-P, WB
Reactivity	Human
Predicted Reactivity	Canine, Guinea pig, Mouse, Porcine, Rat

Related Conjugates & Formulations

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Biotin

FITC

HRP

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunogen is a synthetic peptide directed towards the middle region of human CSF1
Target	CSF1
Molecular Weight	61 kDa
Purification	Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration	Batch dependent within range: 100 μl at 0.5-1 mg/ml
Disclaimer	For research use only

Alternative Names

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anti MCSF antibody, anti MGC31930 antibody, anti CSF-1 antibody

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25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. Peptide is cross-reacting with CSF4 at ~59 kDa, and is also present in isoforms of CSF1 at ~56 kDa and ~51 kDa.

CSF1 in human placenta was detected using HRP/AEC red color stain, Working dilution 10 ug/mL.

Sample Tissue: Human Fetal Liver, Antibody Dilution: 1.0 ug/mL.

Sample Tissue: Human Jurkat Whole Cell, Antibody Dilution: 1 ug/mL.

Sample Tissue: Human Liver Tumor, Antibody Dilution: 1 ug/mL.

Sample Tissue: Human THP-1 Whole Cell, Antibody Dilution: 1 ug/mL.

Sample Type: 721_B, Antibody Dilution: 1.0 ug/mL, CSF1 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.

Positive control (+): Human liver (LI), Negative control (-): MCF7 (N10), Antibody concentration: 3 ug/mL.

WB Suggested Anti-CSF1 Antibody Titration: 0.2-1 ug/mL, ELISA Titer: 1:62500, Positive Control: COLO205 cell lysate.

WB Suggested Anti-CSF1 antibody Titration: 1 ug/mL, Sample Type: Human heart.

Quick Database Links

Gene Symbol

CSF1

UniProt

P09603

RefSeq (Protein):NP_000748

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_000748

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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Immunofluorescence

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ICC

Immunocytochemistry

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