CTLA4 Rabbit Polyclonal Antibody

SKU: orb156486

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Description

Research Area

Autoimmune, CD Markers, Cell Biology, Cytotoxic Cells, Immue System, Immunology, SARS /COVID-19, Stem Cell Research

Images & Validation

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Item 1 of 7

Tested Applications	FC, WB
Dilution Range	WB=1:500-2000, Flow-Cyt=1μg/Test
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Bovine, Canine, Rabbit, Sheep

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human CTLA-4/CD152 (75-170/223aa)
Target	CTLA4
Molecular Weight	21 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% ProClin300 and 50% Glycerol
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CD 152; CD152; CD152 antigen; Celiac disease 3; CELIAC3; CTLA 4; CTLA-4; Cytotoxic T cell associated 4; Cytotoxic T lymphocyte associated 4; Cytotoxic T lymphocyte associated antigen 4; Cytotoxic T lymphocyte associated protein 4; Cytotoxic T lymphocyte associated serine esterase 4; Cytotoxic T lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; Cytotoxic T-lymphocyte-associated protein 4; GSE; IDDM12; CD152 isoform; CTLA4_HUMAN; GRD4; ICOS; Ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4.

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Blank control (blue): Molt-4 Cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-CTLA4/FITC Conjugated antibody, Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/FITC orange), used under the same conditions.

Blank control (blue): Mouse Spleen Cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-Phospho-c-Fos (Thr325)/FITC Conjugated antibody, Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/FITC (orange), used under the same conditions.

Blank control: Hela (blue), the cells were fixed with 2% paraformaldehyde (10 min), Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1X PBS containing 0.5% BSA, Primary Antibody Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA (green).

Blank control: Raji (blue). Primary Antibody: Rabbit Anti-CTLA4 antibody (orb156486), Dilution: 5 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1X PBS containing 0.5% BSA.

Sample: A549 (Human) Cell Lysate at 30 ug, Primary: Anti-CTLA4 (orb156486) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 21 kD, Observed band size: 35 kD.

Sample: Liver (Mouse) Cell Lysate at 30 ug, Primary: Anti-CTLA4 (orb156486) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 21 kD, Observed band size: 35 kD.

Sample: Liver (Rat) Cell Lysate at 30 ug, Primary: Anti-CTLA4 (orb156486) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 21 kD, Observed band size: 35 kD.

Quick Database Links

Gene Symbol

CTLA4

UniProt

P16410

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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