CX3CR1 Rabbit Polyclonal Antibody

SKU: orb10490

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Description

Research Area

Cell Adhesion, Cell Biology, HIV, Host-Virus Interaction, Infectious Disease, Neurology, Nociception, SARS /COVID-19, Virology

Images & Validation

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Item 1 of 4

Tested Applications	FC, IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
Reactivity	Mouse, Rat
Predicted Reactivity	Bovine, Canine, Human, Rabbit

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human CX3CR1 (151-250/355aa)
Target	CX3CR1
Molecular Weight	39 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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beta chemokine receptor-like 1; C X3 C CKR 1; CCRL1; Chemokine C X3 C motif receptor 1; CMK BRL 1; CMKBLR1; CMK-BRL-1; CMKDR1; CX3C chemokine receptor 1; CX3C CKR1; Fractalkine Receptor; GPR13; GPRV28; V28; Chemokine receptor 1(chemokine(C-X3-C motif) receptor 1; C-X3-C CKR-1; CX3C1_HUMAN; CX3CR1; G-protein coupled receptor 13.

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Blank control: Raw264.7. Primary Antibody (green line): Rabbit Anti-CX3CR1 antibody (orb10490), Dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (rat spinal cord), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CX3CR1) Polyclonal Antibody, Unconjugated (orb10490) at 1:1000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Heart (Mouse) Lysate at 40 ug, Heart (Rat) Lysate at 40 ug, Primary: Anti-CX3CR1 (orb10490) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50'55 kD, Observed band size: 53 kD.

Sample: Lane 1: Mouse Spinal cord tissue lysates, Lane 2: Mouse Cerebrum tissue lysates, Lane 3: Rat Spinal cord tissue lysates, Lane 4: Rat Cerebrum tissue lysates, Primary: Anti-CX3CR1 (orb10490) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 39 kDa, Observed band size: 47 kDa.

Quick Database Links

Gene Symbol

CX3CR1

UniProt

P49238

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

Flow Cytometry

Immunofluorescence