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Cytokeratin 5 Rabbit Polyclonal Antibody
SKU: orb10408
Featured
Description
Images & Validation
−Item 1 of 4
| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Bovine, Canine, Equine, Porcine, Rabbit |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human CK5 (331-430/590aa) |
| Target | KRT5 |
| Molecular Weight | 64 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Cytokeratin 5; Keratin, type II cytoskeletal 5; CK-5; CK 5; CK5; Keratin-5; K5; 58 kDa cytokeratin; KRT5; DDD; EBS 2; EBS2; K5; Keratin 5(epidermolysis bullosa simplex Dowling-Meara/Kobner/Weber-Cockayne types); Keratin 5; Keratin Type II Cytoskeletal 5; Keratin5; KRT 5; KRT 5A; KRT5; Cytokeratin-5; Cytokeratin5; DDD; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; K2C5_HUMAN; Keratin 5; Keratin; Keratin Type II Cytoskeletal 5; Keratin5; KRT5A; type II cytoskeletal 5; Type-II keratin Kb5.
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Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Cytokeratin 5) Polyclonal Antibody, Unconjugated (orb10408) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cervical cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Cytokeratin 5) Polyclonal Antibody, Unconjugated (orb10408) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat bladder tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Cytokeratin 5) Polyclonal Antibody, Unconjugated (orb10408) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Sample: Lane 1: Skin (Mouse) Lysate at 40 ug, Lane 2: Uterus (Mouse) Lysate at 40 ug, Lane 3: Urinary bladder (Mouse) Lysate at 40 ug, Lane 4: Esophagus (Mouse) Lysate at 40 ug, Lane 5: Skin (Rat) Lysate at 40 ug, Lane 6: Uterus (Rat) Lysate at 40 ug, Lane 7: Urinary bladder (Rat) Lysate at 40 ug, Lane 8: Esophagus (Rat) Lysate at 40 ug, Primary: Anti-Cytokeratin 5 (orb10408) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 62 kD, Observed band size: 60 kD.
Quick Database Links
Gene Symbol
KRT5
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
Cytokeratin 5 Rabbit Polyclonal Antibody (orb10408)
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