Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human DcR2 (56-160/386aa)
Target	TNFRSF10D
Molecular Weight	36 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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TNFRSF10D; Decoy receptor 2; CD 264; CD264; CD-264; DcR 2; decoy with truncated death domain; ID; TNF receptor related receptor for TRAIL; TNF related apoptosis inducing ligand receptor 4; TNFRSF 10D; TRAIL R4; TRAIL receptor 4; TRAIL receptor with a truncated death domain; TRAILR4; TRIALR 4; TRUNDD; Tumor necrosis factor receptor superfamily member 10d decoy with truncated death domain; Tumor necrosis factor receptor superfamily member 10D precursor; TR10D_HUMAN.

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Blank control: Jurkat cells (blue). Primary Antibody: Rabbit Anti-DcR2 antibody (orb10524), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (orb10524, 1 µg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.

Sample: Lane 1: Human MKN45 cell lysates, Lane 2: Human SW480 cell lysates, Lane 3: Human HUVEC cell lysates, Lane 4: Human DU145 cell lysates, Lane 5: Human HepG2 cell lysates, Primary: Anti-DcR2 (orb10524) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 36 kDa, Observed band size: 50 kDa.

Quick Database Links

Gene Symbol

TNFRSF10D

UniProt

Q9UBN6

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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