DLC1 Rabbit Polyclonal Antibody

SKU: orb312222

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Description

Research Area

Cancer Research, Cell Biology, Ras Family, Tumor Biology, Tumor Suppressors

Images & Validation

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Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Mouse
Predicted Reactivity	Mouse

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human DLC1 (601-700/1528aa)
Target	DLC1
Molecular Weight	171 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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ARHGAP 7; ARHGAP7; Deleted in liver cancer 1; Deleted in liver cancer 1 protein; DLC 1; Dlc-1; DLC1; DLC1 Rho GTPase activating protein; FLJ21120; HP; HP protein; KIAA1723; p122 RhoGAP; RHG07_HUMAN; Rho GTPase activating protein 7; Rho GTPase-activating protein 7; Rho type GTPase activating protein 7; Rho-type GTPase-activating protein 7; StAR related lipid transfer(START) domain containing 12; StAR related lipid transfer protein 12; StAR-related lipid transfer protein 12; STARD 12; StARD12; START domain containing protein 12; START domain-containing protein 12.

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Sample: Heart (Mouse) Lysate at 40 ug, Primary: Anti-DLC1 (orb312222) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 171 kD, Observed band size: 125 kD.

Tissue/Cell: human colon cancer, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-DLC1 Polyclonal Antibody, Unconjugated (orb312222) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

DLC1

UniProt

Q96QB1

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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