EphB2 Rabbit Polyclonal Antibody

SKU: orb10600

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Description

Research Area

Axonal Guidance Proteins, Cell Biology, Eph Eeceptors, Kinases, Neuroscience, Receptor Tyrosne Kinases, Tyrosine Kinase Signaling Pathway

Images & Validation

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Item 1 of 4

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Canine, Gallus

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human EphB2 (101-200/1055aa)
Target	EPHB2
Molecular Weight	114 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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PHB2_HUMAN; Ephrin type-B receptor 2; EC:2.7.10.1; Developmentally-regulated Eph-related tyrosine kinase; ELK-related tyrosine kinase; EPH tyrosine kinase 3; EPH-like kinase 5(EK5; hEK5); Renal carcinoma antigen NY-REN-47; Tyrosine-protein kinase TYRO5; Tyrosine-protein kinase receptor EPH-3; EphB2/CTF1; EphB2/CTF2; DRT; EPHT3; EPTH3; TYRO5; CAPB; PCBC; BDPLT22;

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Sample: Lane 1: Testis (Mouse) Lysate at 40 ug, Lane 2: Testis (Rat) Lysate at 40 ug, Lane 3: Huvec (Human) Cell Lysate at 30 ug, Lane 4: U251 (Human) Cell Lysate at 30 ug, Lane 5: A431 (Human) Cell Lysate at 30 ug, Lane 6: U2os (Human) Cell Lysate at 30 ug, Lane 7: MDA-MB-231 (Human) Cell Lysate at 30 ug, Lane 8: K562 (Human) Cell Lysate at 30 ug, Primary: Anti-EphB2 (orb10600) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 125 kD, Observed band size: 120 kD.

Sample: Testis (Mouse) Lysate at 40 ug, Primary: Anti-EphB2 R (orb10600) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 114 kD, Observed band size: 114 kD.

Tissue/Cell: human colon carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-EphB2 R Polyclonal Antibody, Unconjugated (orb10600) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-EphB2 R Polyclonal Antibody, Unconjugated (orb10600) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

EPHB2

UniProt

P29323

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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