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Ferritin Light Chain Rabbit Polyclonal Antibody
SKU: orb1152031
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Description
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, IF, IHC-Fr, IHC-P |
|---|---|
| Dilution range | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 |
| Reactivity | Human |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant human Ferritin Light Chain (1-175/175aa) |
| Target | FTL |
| Molecular Weight | 19-21 kDa |
| Purification | 0.01M PBS(pH7.4) |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Disclaimer | For research use only |
Alternative Names
−Ferritin light polypeptide; Ferritin L chain; Ferritin L subunit; Ferritin light chain; Ferritin light polypeptide like 3; FRIL; FRIL_HUMAN; FTL; L apoferritin; MGC71996; NBIA 3; NBIA3.
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Measured by its binding ability in a indirect ELISA. Immobilized Human FTL, His Tag at 2 µg/mL (100 µl/well) can bind Anti-Human PSA monoclonal antibody, the minimum detection concentration is 0.5 ng/ml.
Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Ferritin Light Chain) Polyclonal Antibody, Unconjugated at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Quick Database Links
Gene Symbol
FTL
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Ferritin Light Chain Rabbit Polyclonal Antibody (orb1152031)
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