Flag Tag Mouse Monoclonal Antibody (HRP)

SKU: orb526284

Description

The Flag Tag Mouse monoclonal antibody (HRP) is an HRP conjugated monoclonal antibody suitable for ELISA, WB. This product reacts with multiple species.

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, IHC-Fr, IHC-P, WB
Dilution range	WB=1:5000-50000, ELISA=1:5000-10000, IHC-P=1:200-1000, IHC-F=1:200-1000
Reactivity	All

Related Conjugates & Formulations

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Unconjugated

Biotin

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG
Clone No.	3D7
Immunogen	KLH conjugated Flag Tag
Purification	Affinity purified by Protein G
Conjugation	HRP

Storage & Handling

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Storage	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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DDDDK epitope tag; DDDK; ddk; DYKDDDDK; DYKDDDDK epitope tag; DYKDDDDK tag; DYKDDDDK-Tag; ECS epitope tag; ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; FLAG; FLAG-tag.

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Flag Tag Mouse Monoclonal Antibody (HRP)

5 ng rhTNFSF13B-flag protein per lane probed with Flag Tag/HRP monoclonal antibody respectively, unconjugated (orb526284) at 1:50000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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