GAPDHS Rabbit Polyclonal Antibody

SKU: orb159216

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Description

Images & Validation

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Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Mouse, Rat
Predicted Reactivity	Bovine, Canine, Equine, Human, Sheep

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human GAPDHS (251-350/408aa)
Target	GAPDHS
Molecular Weight	45 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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GAPDH2; GAPD2; GAPDH 2; GAPDH 2; GAPDHS; GAPDS; Glyceraldehyde 3 phosphate dehydrogenase spermatogenic; Glyceraldehyde 3 phosphate dehydrogenase, testis specific; HSD 35; HSD35; Spermatogenic cell specific glyceraldehyde 3 phosphate dehydrogenase 2; Spermatogenic glyceraldehyde 3 phosphate dehydrogenase; G3PT_HUMAN; HSD-35.

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Sample: Testis (Mouse) Lysate at 40 ug, Primary: Anti-GAPDHS (orb159216) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45 kD, Observed band size: 47 kD.

Tissue/Cell: rat testis tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-GAPDHS Polyclonal Antibody, Unconjugated (orb159216) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

GAPDHS

UniProt

O14556

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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