GFAP Rabbit Polyclonal Antibody (Cy5)

SKU: orb913879

Description

GFAP Rabbit Polyclonal Antibody (Cy5) is a Cy5 conjugated antibody targeting GFAP. This antibody is suitable for FC, IF. It exhibits reactivity with Human, Mouse, Rat samples.

Research Area

Astrocyte, Astrocyte Markers, Cell Biology, Intermediate Filaments, Neuroglia, Neurology, Neuroscience

Images & Validation

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Tested Applications	FC, IF
Dilution range	Flow-Cyt=1μg/Test, IF=1:200-800
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Bovine, Canine, Porcine, Rabbit, Sheep

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human GFAP (51-150/432aa)
Target	GFAP
Purification	Affinity purified by Protein A
Conjugation	Cy5

Storage & Handling

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Storage	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Astrocyte Marker; FLJ45472; GFAP; Glial Fibrillary Acidic Protein; Intermediate filament protein; GFAP_HUMAN.

Quality Guarantee

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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation: Anti-GFAP /cy5 Antibody, conjugated (orb913879) at 1:200 overnight at 4°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation: Anti-GFAP /cy5 Antibody, conjugated (orb913879) at 1:200 overnight at 4°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (Rat Brian), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation: Anti-GFAP/cy5 Antibody, conjugated (orb913879) at 1:200 overnight at 4°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (Rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation: Anti-GFAP/cy5 Antibody, conjugated (orb913879) at 1:200 overnight at 4°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

GFAP

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