GLI1 Rabbit Polyclonal Antibody

SKU: orb574163

Description

Rabbit polyclonal antibody to GLI1

Images & Validation

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Item 1 of 6

Tested Applications	WB
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat

Related Conjugates & Formulations

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Biotin

FITC

HRP

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunogen is a synthetic peptide directed towards the C terminal region of human GLI1
Target	GLI1
Molecular Weight	118 kDa
Purification	Protein A purified

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration	1.0 mg/ml
Disclaimer	For research use only

Alternative Names

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GLI, PPD1, PAPA8

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WB Suggested Anti-GLI1 Antibody Titration: 2.5 ug/ml, Positive Control: HepG2 cell lysate.

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/ml of the antibody was used in this experiment. Canonical 118 kDa isoform is identified, a second isoform of 114 kDa, and a third isoform of 105 kDa are also present in some samples.

Sample Tissue: Human Jurkat, Antibody dilution: 1.0 ug/ml.

Sample Type: HepG2 Whole Cell lysates, Antibody dilution: 0.5 ug/ml.

Sample Type: Jurkat Whole Cell lysates, Antibody dilution: 5.0 ug/ml.

WB Suggested Anti-GLI1 Antibody Titration: 1.0 ug/ml, Positive Control: HepG2 cell lysate.

Quick Database Links

Gene Symbol

GLI1

UniProt

P08151

RefSeq (Protein):NP_005260

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_005260

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Protocol Information

Western Blot (IB, immunoblot)

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