GLUT1 Rabbit Polyclonal Antibody

SKU: orb10725

This product can be custom made

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Description

GLUT1 Rabbit Polyclonal Antibody

Research Area

Cancer Research, Cardiovascular Research, Diabetes Research, Metabolism

Images & Validation

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Item 1 of 3

Tested Applications	FC, IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Bovine, Canine, Gallus, Porcine, Sheep

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human GLUT1 (191-270/492aa)
Target	SLC2A1
Molecular Weight	52 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CSE; DYT17; DYT18; DYT9; EIG12; GLUT; GLUT-1; GLUT1; GLUT1DS; HTLVR; PED; SDCHCN; GT1; M100200; Rgsc200; GLUTB; GTG1; Gtg3; RATGTG1; GTR1_HUMAN; SLC2A1; Glucose transporter type 1, erythrocyte/brain (GLUT-1); HepG2 glucose transporter; GTR1_MOUSE; Glucose transporter type 1, erythrocyte/brain (GLUT-1 | GT1); GTR1_RAT;

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Mouse splenocytes stained with Rabbit Anti-GLUT1 Polyclonal Antibody, PE conjugated at 1:50.

Sample: Lane 1: Heart (Mouse) Lysate at 40 ug, Lane 2: Cerebrum (Mouse) Lysate at 40 ug, Lane 3: Muscle (Mouse) Lysate at 40 ug, Lane 4: Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-GLUT1 (orb10725) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kD, Observed band size: 52 kD.

Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-GLUT1 Polyclonal Antibody, Unconjugated (orb10725) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

SLC2A1

UniProt

P11166

UniProt Details

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No UniProt data available

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Datasheet

Product Information

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

Flow Cytometry

Immunofluorescence

Filter by Species

Mouse (1)

Srijon K Banerjee 1, Lance R Thurlow 2, Kartik Kannan 1, Anthony R Richardson Glucose transporter 1 is essential for the resolution of methicillin-resistant S. aureus skin and soft tissue infections Cell Rep, (2024)

PubMed: 38990718

Reactivity

Mouse