GPR91 Rabbit Polyclonal Antibody

SKU: orb157370

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Description

Research Area

Cell Biology, G Protein-Coupled Receptors, GPCR Signaling Pathway

Images & Validation

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Item 1 of 5

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Mouse
Predicted Reactivity	Mouse

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human GPR91 (131-334/334aa)
Target	SUCNR1
Molecular Weight	39 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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G protein coupled receptor 91; G-protein coupled receptor 91; GPR 91; P2Y purinoceptor 1; P2Y purinoceptor 1-like; Purinergic Receptor P2U2; Succinate receptor; Succinate receptor 1; SUCN R1; SUCNR 1; SUCNR1; SUCR1_HUMAN.

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Protein: 1.kidney lyates (mo), 2.liver lyates (mo), Primary: Rabbit Anti-GPR91 (orb157370) at 1:300, Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG (H+L) at 1: 10000, Predicted band size: 39 kD, Observed band size: 49 kD.

Sample: HepG2 Cell (Human) Lysate at 40 ug, Primary: Anti-GPR91 (orb157370) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 39kD, Observed band size: 39kD.

Tissue/Cell: human breast cancer, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-GPR91 Polyclonal Antibody, Unconjugated (orb157370) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-GPR91 Polyclonal Antibody, Unconjugated (orb157370) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Western blot analysis of extracts from Liver tissue (mo) using GPR91 Antibody.

Quick Database Links

Gene Symbol

SUCNR1

UniProt

Q9BXA5

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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Filter by Applications

IF (1)
IHC (1)
WB (2)

Filter by Species

Mouse (2)

W Su, Y Wang, C Zu, L Lei, H Li G protein-coupled receptor 91 promotes the inflammatory response to Porphyromonas gingivalis in bone marrow-derived macrophages Heliyon, (2024)

PubMed:

Applications

IF, IHC, WB

Reactivity

Mouse

W Su, D Zhang, Y Wang, L Lei, H Li G protein-coupled receptor 91 activations suppressed mineralization in Porphyromonas gingivalis–infected osteoblasts researchsquare.com, (2024)

PubMed:

Applications

Reactivity

Mouse