GPX4 Rabbit Polyclonal Antibody

SKU: orb5346

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Description

Research Area

Antioxidants, Cancer Research, Cell Biology, Metabolism, Mitochondrial, Mitochondrial Markers

Images & Validation

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Item 1 of 3

Tested Applications	WB
Dilution Range	WB=1:500-2000
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Bovine, Porcine, Sheep

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human Glutathione Peroxidase 4 (101-197/197aa)
Target	GPX4
Molecular Weight	22 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Glutathione peroxidase 4; GPX 4; GPX-4; GPX4; GPX4_HUMAN; GSHPx-4; MCSP; mitochondrial; PHGPx; phospholipid hydroperoxidase; phospholipid hydroperoxide glutathione peroxidase; phospholipid hydroperoxide glutathione peroxidase; phospholipid hydroperoxide glutathione peroxidase mitochondrial; snGPx; snPHGPx; Sperm nucleus glutathione peroxidase.

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Sample: Kidney (mouse) Lysate at 40 ug, Primary: Anti-GPX4 (orb5346) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22kD, Observed band size: 20 kD.

Sample: Lane 1: Testis (Mouse) Tissue Lysate at 40 ug, Lane 2: Liver (Mouse) Tissue Lysate at 40 ug, Lane 3: Kidney (Mouse) Tissue Lysate at 40 ug, Lane 4: Heart (Mouse) Tissue Lysate at 40 ug, Lane 5: Testis (Rat) Tissue Lysate at 40 ug, Lane 6: Liver (Rat) Tissue Lysate at 40 ug, Lane 7: Kidney (Rat) Tissue Lysate at 40 ug, Lane 8: Heart (Rat) Tissue Lysate at 40 ug, Lane 9: U937 (Human) Cell Lysate at 30 ug, Lane 10: HepG2 (Human) Cell Lysate at 30 ug, Lane 11: MCF-7 (Human) Cell Lysate at 30 ug, Lane 12: Huvec (Human) Cell Lysate at 30 ug, Lane 13: 293T (Human) Cell Lysate at 30 ug, Primary: Anti-GPX4 (orb5346) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22 kD, Observed band size: 20-22 kD.

Sample: Testis (Mouse) Lysate at 40 ug, Primary: Anti-GPX4 (orb5346) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22 kD, Observed band size: 19 kD.

Quick Database Links

Gene Symbol

GPX4

UniProt

P36969

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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