Hsp27/HSPB1 Rabbit Polyclonal Antibody (Carrier-free)

SKU: orb3078330

Description

The Hsp27/HSPB1 Rabbit Polyclonal Antibody (Carrier-free) is a Carrier-free antibody raised in rabbit. It is immunogen affinity purified and suitable for use in FC, ICC, IF, IHC, IHC-Fr, IP, and WB applications. This antibody targets the Heat shock protein beta-1 in Human samples.

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Neuroscience, Protein Biochemistry, Signal Transduction

Images & Validation

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Tested Applications	FC, ICC, IF, IHC, IHC-Fr, IP, WB
Dilution Range	Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, - Immunocytochemistry/Immunofluorescence, 2μg/ml, Human, - Immunoprecipitation, 0.5-2 μg/ml, Human Flow Cytometry (Fixed), 1-3μg/mlx10^6 cells, Human
Reactivity	Human
Application Notes	For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Related Conjugates & Formulations

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Unconjugated

Biotin

HRP

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	E.coli-derived human Hsp27 recombinant protein (Position: M1-K205). Human Hsp27 shares 83% amino acid (aa) sequence identity with mouse Hsp27.
Target	Heat shock protein beta-1
Molecular Weight	27 kDa
Purification	Immunogen affinity purified.
Conjugation	Carrier-free

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Heat shock protein beta 1; HSP 27; Hsp25; HSP27; HSP28; HSPB1

Quality Guarantee

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