His tag Mouse Monoclonal Antibody

SKU: orb500509

Description

Research Area

6x his tag, His Tag

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, FC, ICC, IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:5000-50000, ELISA=1:1000-5000, IHC-P=1:200-1000, IHC-F=1:200-1000, IF=1:200-1000, Flow-Cyt=1ug/Test, ICC/IF=1:100-500
Reactivity	All

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG
Clone No.	6E6
Immunogen	KLH conjugated HHHHHH peptide
Purification	Affinity purified by Protein G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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HisX6; 6His; His-Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine.; His Tag;

Quality Guarantee

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Sample: His-Tagged Fusion Protein Overexpression E.coli Lysate, Primary: Anti-His tag (orb500509) at 1/200000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 41 kDa, Observed band size: 45 kDa.

Sample: Lane 1: His-Tagged Fusion Protein Overexpression E.coli Lysate (orb1516792) at 2 ug Lane 2: His-Tagged Fusion Protein Overexpression E.coli Lysate (orb1516792) at 2 ug Primary: Anti-His tag (orb500509) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 45 kD.