HSP60 Rabbit Polyclonal Antibody

SKU: orb10846

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Description

Research Area

Neuroscience

Images & Validation

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Item 1 of 5

Tested Applications	ICC, IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Bovine, Canine, Equine, Rabbit

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human HSP60 (501-573/573aa)
Target	HSPD1
Molecular Weight	58 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CPN60; GROEL; HLD4; HSP-60; HSP60; HSP65; HuCHA60; SPG13; 60kDa; HSP-65; Hspd1-30p; CH60_MESAU; HSPD1; 60 kDa chaperonin; Chaperonin 60 (CPN60); Heat shock protein 60 (HSP-60 | Hsp60); Mitochondrial matrix protein P1; 5.6.1.7; CH60_HUMAN; Heat shock protein family D member 1; P60 lymphocyte protein; CH60_MOUSE; CH60_RAT;

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HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (HSP60) polyclonal Antibody, Unconjugated (orb10846) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HSP60) Polyclonal Antibody, Unconjugated (orb10846) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Lane 1: Liver (Mouse) Lysate at 40 ug, Lane 2: Adrenal gland (Mouse) Lysate at 40 ug, Lane 3: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 4: Adrenal gland (Rat) Lysate at 40 ug, Lane 5: Jurkat (Human) Cell Lysate at 30 ug, Lane 6: MDA-MB-231 (Human) Cell Lysate at 30 ug, Lane 7: HepG2 (Human) Cell Lysate at 30 ug, Lane 8: Hela (Human) Cell Lysate at 30 ug, Lane 9: K562 (Human) Cell Lysate at 30 ug, Primary: Anti-HSP60 (orb10846) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 62 kD, Observed band size: 62 kD.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-HSP60 Polyclonal Antibody, Unconjugated (orb10846) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-HSP60 Polyclonal Antibody, Unconjugated (orb10846) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

HSPD1

UniProt

P10809

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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ICC

Immunocytochemistry

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