HSP90 alpha Mouse Monoclonal Antibody

SKU: orb500766

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Description

Research Area

Cancer Research, Signal Transduction, Tumor Biomarkers

Images & Validation

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Item 1 of 6

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Mouse, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG
Clone No.	3H7
Immunogen	Recombinant human HSP90 alpha Protein (549-692/732aa)
Target	HSP90AA1
Molecular Weight	81 kDa
Purification	Affinity purified by Protein G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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HSP-90 alpha; HSP 86; HSP 86; Renal carcinoma antigen NY REN 38; Heat shock 86 kDa; Heat shock 90kDa protein 1 alpha; Heat shock protein 90kDa alpha(cytosolic) class A member 1; heat shock protein 90kDa alpha(cytosolic), class A member 2; Heat shock protein HSP 90-alpha; HS90A_HUMAN; HSP 86; HSP86; Hsp89; HSP89A; HSP90A; HSP90AA1; HSP90ALPHA; HSP90N; HSPC1; HSPCA; HSPCAL1; HSPCAL3; HSPCAL4; HSPN; LAP2; Renal carcinoma antigen NY-REN-38.

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Paraformaldehyde-fixed, paraffin embedded (Human colon cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HSP90 alpha) Monoclonal Antibody, Unconjugated (orb500766) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HSP90 alpha) Monoclonal Antibody, Unconjugated (ascites of orb500766 3H7) at 1:2000 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HSP90 alpha) Monoclonal Antibody, Unconjugated (orb500766) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: 293T (Human) Cell Lysate at 30 ug, NIH/3T3 (Mouse) Cell Lysate at 40 ug, Primary: Anti-HSP90 alpha (orb500766) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 81 kD, Observed band size: 81 kD.

Sample: Hela Cell (Human) Lysate at 40 ug, NIH/3T3 Cell (Mouse) Lysate at 40 ug, Primary: Anti-HSP90 alpha (orb500766) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 81 kD, Observed band size: 98 kD.

Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Mouse Cerebrum tissue lysates, Lane 3: Mouse Placenta tissue lysates, Lane 4: Rat Placenta tissue lysates, Lane 5: Human U251 cell lysates, Lane 6: Human Molt-4 cell lysates, Lane 7: Human HL60 cell lysates, Lane 8: Human A549 cell lysates, Primary: Anti-HSP90 alpha (orb500766) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 81 kD, Observed band size: 81 kD.

Quick Database Links

Gene Symbol

HSP90AA1

UniProt

P07900

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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