HSP90 beta Mouse Monoclonal Antibody

SKU: orb500620

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Description

Research Area

Signal Transduction

Images & Validation

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Item 1 of 2

Tested Applications	WB
Dilution range	WB=1:500-1000
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Mouse, Rat

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG
Clone No.	2A2
Immunogen	KLH conjugated synthetic peptide derived from human HSP90 beta
Target	HSP90AB1
Molecular Weight	84 kDa
Purification	Affinity purified by Protein G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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HSP90 beta; D6S182; FLJ26984; Heat shock 90kD protein 1, beta; Heat shock protein 90kDa alpha(cytosolic) class B member 1; Heat shock protein beta; Heat shock protein HSP 90 beta; HSP 84; HSP84; HSP 90; HSP-90 BETA; HSP90B; HSPC2; HSPCB; HS90B_HUMAN; Heat shock protein HSP 90-beta; Heat shock 84 kDa.

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Sample: Hela KO HSP90 beta (Human) Cell Lysate at 30 ug, Hela (Human) Cell Lysate at 30 ug, Primary: Anti-HSP90 beta (orb500620) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse t 1/20000 dilution, Predicted band size: 84 kD, Observed band size: 84 kD.

Sample: Lane 1: A431 (Human) Cell Lysate at 30 ug, Lane 2: Hela (Human) Cell Lysate at 30 ug, Lane 3: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 4: K562 (Human) Cell Lysate at 30 ug, Lane 5: HepG2 (Human) Cell Lysate at 30 ug, Lane 6: U2os (Human) Cell Lysate at 30 ug, Lane 7: A549 (Human) Cell Lysate at 30 ug, Lane 8: HL60 (Human) Cell Lysate at 30 ug, Lane 9: Jurkat (Human) Cell Lysate at 30 ug, Lane 10: Testis (Mouse) Lysate at 40 ug, Lane 11: Testis (Rat) Lysate at 40 ug, Lane 12: Cerebrum (Mouse) Lysate at 40 ug, Lane 13: Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-HSP90 beta (orb500620) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 90 kD, Observed band size: 86 kD.

Quick Database Links

Gene Symbol

HSP90AB1

UniProt

P08238

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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