Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1, k
Clone No.	HI30
Immunogen	Recombinant human CD45
Target	PTPRC
Molecular Weight	180-220 kDa
Purification	Affinity purified by Protein G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4).
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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B220; CD45; CD45R; GP180; IMD105; L-CA; LCA; LY5; T200; Ly-5; Lyt-4; loc; RT7; PTPRC_HUMAN; PTPRC; Leukocyte common antigen (L-CA); 3.1.3.48; PTPRC_MOUSE; Lymphocyte antigen 5 (Ly-5); PTPRC_RAT;

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Blank control: Molt4. Primary Antibody (green line): Mouse Anti-CD45 antibody (orb526577), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Mouse IgG. Secondary Antibody: Goat anti-mouse IgG-AF488, dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

PTPRC

UniProt

P08575

UniProt Details

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No UniProt data available

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Protocol Information

Flow Cytometry

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