IGFBP5 Rabbit Polyclonal Antibody

SKU: orb10885

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Description

Research Area

Cancer Research, Insulin and Insulin Like, Signal Transduction

Images & Validation

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Item 1 of 3

Tested Applications	WB
Dilution range	WB=1:500-2000
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Canine, Equine, Gallus, Porcine, Rat, Sheep

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human IGFBP5 (101-200/272aa)
Target	IGFBP5
Molecular Weight	30 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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IBP 5; IBP5; IGF binding protein 5; IGF BP5; Insulin Like Growth Factor Binding Protein 5; IBP5_HUMAN; Insulin-like growth factor-binding protein 5; IBP-5; IGF-binding protein 5; IGFBP-5.

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Sample: Kidney (Mouse) Lysate at 40 ug, Primary: Anti-IGFBP5 (orb10885) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 30 kD, Observed band size: 30 kD.

Tissue/Cell: human breast carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-IGFBP5 Polyclonal Antibody, Unconjugated (orb10885) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: human colon carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-IGFBP5 Polyclonal Antibody, Unconjugated (orb10885) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

IGFBP5

UniProt

P24593

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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