Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from rat IL-1 Alpha (155-230/270aa)
Target	IL1A
Molecular Weight	17, 31 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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interleukin-1 alpha; Hematopoietin 1; IL 1 alpha; IL1 alpha; IL 1; IL 1A; Il-1a; IL1; IL1A; IL1F1; ilia; Interleukin 1 alpha; Interleukin 1 alpha precursor; Interleukin1 alpha; Preinterleukin 1 alpha; Pro interleukin 1 alpha; Prointerleukin 1 alpha; IL1A_HUMAN.

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Blank control: Raw264.7. Primary Antibody (green line): Rabbit Anti-IL-1 Alpha antibody (orb157678), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-IL-1 Alpha (orb157678) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 17/31 kD, Observed band size: 31 kD.

Sample: Lver (Mouse) Lysate at 40 ug, Primary: Anti-IL-1 Alpha (orb157678) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 17/31 kD, Observed band size: 31 kD.

Quick Database Links

Gene Symbol

IL1A

UniProt

P16598

UniProt Details

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No UniProt data available

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Western Blot (IB, immunoblot)

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