IL-23 Rabbit Polyclonal Antibody

SKU: orb10907

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Description

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
Reactivity	Human, Mouse
Predicted Reactivity	Rat

Related Conjugates & Formulations

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Biotin

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human IL-23
Target	Il23a
Molecular Weight	21 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Interleukin-23 subunit alpha; Il23a; IL 23; IL 23A; IL23P19; Interleukin 23 alpha subunit p19; JKA3 induced upon T cell activation; P19; SGRF; IL-23 subunit alpha; IL-23-A; IL-23p19; IL23; Interleukin 23 p19 subunit; Interleukin-23 subunit p19; JKA3 induced upon T cell activation; MGC79388; IL23A_HUMAN.

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Sample: Lane 1: Mouse Ear tissue lysates, Lane 2: Human A431 cell lysates, Lane 3: Human Jurkat cell lysates, Primary: Anti-IL-23 (orb10907) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 21 kDa, Observed band size: 21 kDa.

Tissue/Cell: human rhinopolypus tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-IL-23 Polyclonal Antibody, Unconjugated (orb10907) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

Il23a

UniProt

Q9NPF7

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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IHC (1)

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Mouse (1)

A Wojtkowiak-Giera 1, D Kosik-Bogacka 2, N Łanocha-Arendarczyk 3, A Kolasa 4, K Kot 2, P Solarczyk 1, M Derda Toll-like receptors and inflammatory cytokines in the skin of Acanthamoeba spp. infected immunocompetent and immunosuppressed mice Exp Parasitol ., (2025)

PubMed: 40274043

Applications

IHC

Reactivity

Mouse