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ITGB3 Rabbit Polyclonal Antibody

SKU: orb10946

Description

ITGB3 Rabbit Polyclonal Antibody

Research Area

Neuroscience

Images & Validation

Tested ApplicationsIF, IHC-Fr, IHC-P, WB
Dilution RangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
ReactivityHuman, Mouse
Predicted ReactivityBovine, Porcine, Rabbit

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human Integrin beta 3 (27-120/788aa)
TargetITGB3
Molecular Weight90 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

BDPLT16; BDPLT2; BDPLT24; CD61; FMAIT1; GP3A; GPIIIa; GT; GT2; INGRB3; ITB3_HUMAN; ITGB3; Platelet membrane glycoprotein IIIa (GPIIIa); ITB3_MOUSE;

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ITGB3 Rabbit Polyclonal Antibody

Sample: Lane 1: Mouse Bone Lysates, Lane 2: Mouse Kidney Lysates, Primary: Anti-Integrin beta 3 (orb10946) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 84kDa, Observed band size: 84kDa.

ITGB3 Rabbit Polyclonal Antibody

Tissue/Cell: human rectal carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Integrin beta 3/CD61 Polyclonal Antibody, Unconjugated (orb10946) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
IF
Immunofluorescence
View Protocol

ITGB3 Rabbit Polyclonal Antibody (orb10946)

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Available Sizes

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50 μl
¥ 3,250.00
100 μl
¥ 5,070.00
200 μl
¥ 6,110.00
DispatchUsually dispatched within 5-10 working days
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