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JAK1 Rabbit Polyclonal Antibody
SKU: orb10947
Featured
Description
Research Area
Cell Biology, JAK/ STAT Signaling Pathway, Kinases, Signal Transduction, Tyrosine Kinases
Images & Validation
−Item 1 of 4
| Tested Applications | FC, WB |
|---|---|
| Dilution Range | WB=1:500-2000, Flow-Cyt=1ug/Test |
| Reactivity | Human |
| Predicted Reactivity | Rat |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human JAK1 (901-1135/1135aa) |
| Target | JAK1 |
| Molecular Weight | 133 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−JAK 1; JAK 1A; JAK 1B; JAK1; JAK1A; JAK1B; Janus kinase 1(a protein tyrosine kinase); Janus kinase 1; JTK3; Tyrosine protein kinase JAK 1; Tyrosine protein kinase JAK1; JAK1_HUMAN.
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Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Blank control: Jurkat. Primary Antibody (green line): Rabbit Anti-JAK1 antibody (orb10947), Dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Sample: Lovo (Human) Cell Lysate at 30 ug, Siha (Human) Cell Lysate at 30 ug, Primary: Anti-JAK1 (orb10947) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 133 kD, Observed band size: 120 kD.
Sample: Lymph node (Mouse) Lysate at 40 ug, Primary: Anti-JAK1 (orb10947) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 133 kD, Observed band size: 133 kD.
Sample: Thymus (Mouse) Lysate at 30 ug, Primary: Anti-JAK1 (orb10947) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 133 kD, Observed band size: 133 kD.
Quick Database Links
Gene Symbol
JAK1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
JAK1 Rabbit Polyclonal Antibody (orb10947)
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