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Description
Research Area
Signal Transduction
Images & Validation
−Item 1 of 5
| Tested Applications | FC, ICC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:200-800, IHC-F=1:200-800, ICC/IF=1:100-500, IF=1:200-800, Flow-Cyt=1ug/Test |
| Reactivity | Human, Mouse, Rat |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human Ki67 |
| Target | MKI67 |
| Molecular Weight | 320/359 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−KIA; MIB-; MIB-1; PPP1R105; KI67_HUMAN; MKI67; Antigen identified by monoclonal antibody Ki-67 (Antigen KI-67 | Antigen Ki67); marker of proliferation Ki-67; antigen identified by monoclonal antibody Ki-67; protein phosphatase 1, regulatory subunit 105; Molecular Immunology Borstel antibody 1
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4% Paraformaldehyde-fixed Hela (H) cell, Triton X-100 at r.t. for 20 min, Antibody incubation with (Ki-67) polyclonal Antibody, unconjugated (orb13523) 1:100, 90 min at 37°C, followed by conjugated Goat Anti-Rabbit IgG antibody (green, orb868805) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Ki-67) Polyclonal Antibody, Unconjugated (orb13523) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Ki-67) Polyclonal Antibody, Unconjugated (orb13523) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human ovarian serous carcinoma, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki-67 Polyclonal Antibody, Unconjugated (orb13523) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki-67 Polyclonal Antibody, Unconjugated (orb13523) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Quick Database Links
Gene Symbol
MKI67
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
Filter by Applications
Filter by Species
Jianli Liu, Junlin Zhou, Jie Li, Lingyan Zhang, Peili Zhang, Bin Liu Evaluation of rat C6 malignant glioma using spectral computed tomography Experimental and Therapeutic Medicine, 14, 1037 (2017)
Applications
IHC
Reactivity
Rat
Liang, Guodong et al. Silencing of CEMIP suppresses Wnt/β-catenin/Snail signaling transduction and inhibits EMT program of colorectal cancer cells Acta Histochem, 120, 56-63 (2018)
Ki-67 Rabbit Polyclonal Antibody (orb13523)
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