LC3 Rabbit Polyclonal Antibody

SKU: orb500748

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Description

Images & Validation

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Item 1 of 2

Tested Applications	WB
Dilution range	WB=1:500-2000
Reactivity	Mouse
Predicted Reactivity	Bovine, Equine, Human, Porcine, Rat

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human LC3 (31-121/121aa)
Target	MAP1LC3A
Molecular Weight	14/16 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Autophagy related protein LC3 A; Autophagy related protein LC3 B; Autophagy related ubiquitin like modifier LC3 A; Autophagy related ubiquitin like modifier LC3 B; MAP1 light chain 3 like protein 1; MAP1 light chain 3 like protein 2; MAP1A/1B light chain 3 A; MAP1A/1B light chain 3 B; MAP1A/1BLC3; MAP1A/MAP1B LC3 A; MAP1A/MAP1B LC3 B; MAP1ALC3; MAP1BLC3; MAP1LC3A; MAP1LC3B; Microtubule associated protein 1 light chain 3 alpha; Microtubule associated protein 1 light chain 3 beta; Microtubule associated proteins 1A/1B light chain 3; Microtubule associated proteins 1A/1B light chain 3A; Microtubule associated proteins 1A/1B light chain 3B; MLP3A_HUMAN.

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Protein: mouse brain lysates at 40 ug, Primary: rabbit Anti-LC3 (orb500748) at 1:300, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000, Predicted band size: 14 kD Observed band size: 14, 16 kD.

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Cerebellum (Mouse) Lysate at 40 ug, Lane 3: Testis (Mouse) Lysate at 40 ug, Primary: Anti-LC3 (orb500748) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 17/14 kD, Observed band size: 17 kD.

Quick Database Links

Gene Symbol

MAP1LC3A

UniProt

Q9H492

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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WB (1)

Filter by Species

Mouse (1)

Sharmistha Samanta , Gopinath Mukherjee , Sreya Chattopadhyay , Biswadev Bishay Concomitant inhibition of TLR4 and NLRP3 inflammasome in macrophages regulates lipopolysaccharide induced inflammation The Microbe, (2025)

PubMed:

Applications

Reactivity

Mouse