MBP Rabbit Polyclonal Antibody

SKU: orb11027

Featured

Description

Research Area

Neuroglia, Neurology, Neuroscience, Oligodendrocyte Marker

Images & Validation

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Item 1 of 16

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:1000-5000, IHC-P=1:500-2000, IHC-F=1:500-2000, IF=1:500-2000
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Bovine, Canine, Equine, Porcine, Rabbit, Sheep

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from Gpig MBP (69-85/167aa)
Target	MBP
Molecular Weight	33 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Hmbpr; golli-mbp; jve; mld; shi; Mbps; MBP_HUMAN; MBP; Myelin A1 protein; Myelin membrane encephalitogenic protein; MBP_MOUSE; MBP_PIG; MBP_RABIT; Myelin P1 protein; MBP_RAT;

Quality Guarantee

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25 ug total protein per lane of various lysates probed with MBP polyclonal antibody, unconjugated (orb11027) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Blank control: A549. Primary Antibody (green line): Rabbit Anti-MBP antibody (orb11027) Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE Dilution: 1 µg/Test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 20% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: A549. Primary Antibody (green line): Rabbit Anti-MBP antibody (orb11027), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: U937. Primary Antibody (green line): Rabbit Anti-MBP antibody (orb11027), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, Dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded Human Cerebellum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with MBP Polyclonal Antibody, Unconjugated (orb11027) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose red, orb868605), DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded Human Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with MBP Polyclonal Antibody, Unconjugated (orb11027) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with MBP Polyclonal Antibody, Unconjugated (orb11027) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded Human Left Parietal Lobe, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with MBP Polyclonal Antibody, Unconjugated (orb11027) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose red, orb868605), DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with MBP Polyclonal Antibody, Unconjugated (orb11027) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with MBP Polyclonal Antibody, Unconjugated (orb11027) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose red, orb868605), DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with MBP Polyclonal Antibody, Unconjugated (orb11027) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with MBP Polyclonal Antibody, Unconjugated (orb11027) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose red, orb868605), DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with MBP Polyclonal Antibody, Unconjugated (orb11027) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with MBP Polyclonal Antibody, Unconjugated (orb11027) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose red, orb868605), DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with MBP Polyclonal Antibody, Unconjugated (orb11027) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with MBP Polyclonal Antibody, Unconjugated (orb11027) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

MBP

UniProt

P81558

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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Filter by Applications

IHC (1)

Filter by Species

Mouse (1)

Maryam Azimzadeh, Shima Shapoori, Morteza Jafarinia Synergistic effects of melatonin and curcumin in ameliorating experimental autoimmune encephalomyelitis: insights into antiinflammatory, antioxidant, and neuroprotective mechanisms Inflammopharmacology, 33, 7373–7386 (2025)

PubMed: 41207930

Applications

IHC

Reactivity

Mouse