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MOG Rabbit Polyclonal Antibody
SKU: orb11068
Featured
Description
Images & Validation
−Item 1 of 6
| Tested Applications | WB |
|---|---|
| Dilution range | WB=1:500-2000 |
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Guinea pig, Human, Porcine |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from mouse MOG (35-55/247aa) |
| Target | MOG |
| Molecular Weight | 24 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−MOG_HUMAN; Myelin-oligodendrocyte glycoprotein; Myelin oligodendrocyte glycoprotein; BTN6; BTNL11; MOGIG2; NRCLP7;
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (MOG) Polyclonal Antibody, Unconjugated (orb11068) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (MOG) Polyclonal Antibody, Unconjugated (orb11068) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-Myelin-oligodendrocyte glycoprotein (orb11068) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 24 kD, Observed band size: 26 kD.
Sample: Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-Myelin-oligodendrocyte glycoprotein (orb11068) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 24 kD, Observed band size: 26 kD.
Sample: Lane 1: Mouse Spinal cord tissue lysates, Lane 2: Rat Spinal cord tissue lysates, Primary: Anti-MOG (orb11068) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 24 kDa, Observed band size: 26 kDa.
Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-MOG Polyclonal Antibody, Unconjugated (orb11068) 1:200, overnight at 4⒉C, followed by conjugation to the secondary antibody and DAB staining.
Quick Database Links
Gene Symbol
MOG
UniProt
UniProt Details
− No UniProt data available
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Devanjan Dey, Sagar Tyagi, Vadanya Shrivastava, Sweety Rani, Jai Bhagwan Sharma,Subrata Sinha,Jayanth Kumar Palanichamy,Pankaj Seth,Sudip Sen Using Human Fetal Neural Stem Cells to Elucidate the Role of the JAK-STAT Cell Signaling Pathway in Oligodendrocyte Differentiation In Vitro Mol Neurobiol, (2024)
Applications
WB
Reactivity
Human
MOG Rabbit Polyclonal Antibody (orb11068)
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