mScarlet Antibody

SKU: orb1463262

Description

Goat polyclonal antibody to mScarlet (Scarlet fluorescent protein). mScarlet is a basic (constitutively fluorescent), monomeric engineered derivate of red fluorescent protein (RFP) isolated from members of the Discosoma (mushroom coral) family. mScarlet is a ~26.5 kDa protein that is optimally excited at a 569 nm and has a maximum of emission at 594 nm. It is used in research as a reporter to label and study the biology of the cell using a wide range of applications.

Images & Validation

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Tested Applications	IEM, IF, IHC-Fr, IHC-P, WB
Dilution range	WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500
Application Notes	The antibody solution should be gently mixed before use.

Key Properties

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Antibody Type	Primary Antibody
Host	Goat
Clonality	Polyclonal
Isotype	IgG
Immunogen	Antigen: Purified recombinant fluorescent protein produced in E. coli.
Target	In 293HEK cells transfected with cds plasmid detects a band of 26.5 kDa by Western blot. This antibody does not recognize GFP (green fluorescent protein)
Purification	Epitope affinity purified
Conjugation	Unconjugated

Storage & Handling

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Storage	For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours
Buffer/Preservatives	PBS, 20% glycerol and 0.05% sodium azide
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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red fluorescent protein antibody.

Quality Guarantee

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Immunofluorescence hCEC cells lysate using mScarlet antibody

WB analysis of HEK293 transfected cell lysates at 50 µg per lane using mScarlet antibody

Immunofluorescence analysis of Drosophila larvae NMJ muscle 6/7 expressing mScarlet in neurons (DyGlutmScarlet) using mScarlet antibody

Immunofluorescence analysis of hCEC cells using mScarlet antibody

Quick Database Links

Gene Symbol

In 293HEK cells transfected with cds plasmid detects a band of 26.5 kDa by Western blot. This antibody does not recognize GFP (green fluorescent protein)

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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