Neurotrophin 4 Rabbit Polyclonal Antibody

SKU: orb11172

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Description

Images & Validation

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Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Rat
Predicted Reactivity	Mouse

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human NT-4 (121-210/210aa)
Target	NTF4
Molecular Weight	14/27 kDa
Purification	Affinity purified by Protein A

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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GLC1O; Neurotrophic factor 4; Neurotrophic factor 5; Neurotrophin 4/5; Neurotrophin 5(neurotrophin 4/5); Neurotrophin 5; Neurotrophin-4; Neurotrophin-5; Neurotrophin4; Neurotrophin4/5; Neurotrophin5; Neutrophic factor 4; Neutrophic factor 5; NT 4; NT-4; NT 4/5; NT-4/5; NT 5; NT-5; NT4; NT4/5; NT4P; NT5; NTF 4; NTF 5; NTF4; NTF4_HUMAN; NTF5

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Neurotrophin 4 Rabbit Polyclonal Antibody

Sample: DU145 Cell (Human) Lysate at 40 ug, Primary: Anti-Neurotrophin 4 (orb11172) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 14/27 kD, Observed band size: 26 kD.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Neurotrophin 4/NT-4/NT-5 Polyclonal Antibody, Unconjugated (orb11172) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

NTF4

UniProt

P34130

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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