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Description
Research Area
Images & Validation
−| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:200-500, IHC-F=1:200-500, IF=1:200-500 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone No. | B5J1 |
| Immunogen | Recombinant Human NF-L Protein (1-543/543aa) |
| Target | NEFL |
| Molecular Weight | 68 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M PBS (pH7.4). |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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−NF-L Mouse Monoclonal Antibody [orb1291513]
IF, IHC-Fr, IHC-P
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Sample: Lane 1: Human U251 cell lysates, Primary: Anti-NF-L (orb1499409) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 68 kDa, Observed band size: 68 kDa.
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NF-L Mouse Monoclonal Antibody (orb1499409)
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