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Description
Research Area
Apoptosis, Cell Cycle
Images & Validation
−Item 1 of 1
| Tested Applications | IF, IHC-Fr, IHC-P |
|---|---|
| Dilution Range | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Reactivity | Mouse |
| Predicted Reactivity | Bovine, Canine, Human, Rabbit, Rat |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from mouse Nucleolar protein 3/Apoptosis repressor with CARD (1-100/220aa) |
| Target | NOL3 |
| Molecular Weight | 25 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ARC; FCM; MYOCL1; MYP; NOP; NOP30; B430311C09Rik; MYC; NOL3_HUMAN; NOL3; Apoptosis repressor with CARD; Muscle-enriched cytoplasmic protein (Myp); Nucleolar protein of 30 kDa (Nop30); NOL3_MOUSE; NOL3_RAT;
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Tissue/cell: mouse kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Nucleolar protein 3 Polyclonal Antibody, Unconjugated (orb100019) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Quick Database Links
Gene Symbol
NOL3
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
Nucleolar protein 3 Rabbit Polyclonal Antibody (orb100019)
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