OAT1 / SLC22A6 Rabbit Polyclonal Antibody

SKU: orb500999

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Description

OAT1 / SLC22A6 Rabbit Polyclonal Antibody

Images & Validation

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Item 1 of 4

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Mouse
Predicted Reactivity	Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human OCT-1 (285-550/550aa)
Target	SLC22A6
Molecular Weight	62 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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HOAT1; OAT1; PAHT; ROAT1; NKT; Orctl1; mOat1; S22A6_HUMAN; SLC22A6; Organic anion transporter 1 (hOAT1); PAH transporter (hPAHT); Renal organic anion transporter 1 (hROAT1); S22A6_MOUSE; Kidney-specific transport protein; Novel kidney transcript (mNKT); Organic anion transporter 1 (mOAT1); Renal organic anion transporter 1 (mROAT1); S22A6_RAT; Organic anion transporter 1 (rOAT1); renal organic anion transporter 1 (rROAT1);

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OAT1 / SLC22A6 Rabbit Polyclonal Antibody

Sample: Kidney (Human) lysate at 30 ug, Brain (Rat) lysate at 30 ug, Primary: Anti-OAT-1/SLC22A6 (human) (orb500999) at 1:200 dilution, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:3000 dilution, Predicted band size: 62kD, Observed band size: 61kD.

Sample: Liver (Mouse) Lysate at 40 ug, Primary: Anti-SLC22A6 (orb500999) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 62 kD, Observed band size: 62 kD.

Sample: Raji Cell lysate at 30 ug, Primary: Anti-SLC22A6 (orb500999) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000 dilution, Predicted band size: 62 kD, Observed band size: 62 kD.

Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-OAT-1/SLC22A6 (human) Polyclonal Antibody, Unconjugated (orb500999) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

SLC22A6

UniProt

Q4U2R8

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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