PAFAH1B2 Rabbit Polyclonal Antibody

SKU: orb1109

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Description

Research Area

Cardiovascular Research, Cell Biology, Lipid Metabolism

Images & Validation

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Item 1 of 1

Tested Applications	IF, IHC-Fr, IHC-P
Dilution Range	IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Rat
Predicted Reactivity	Equine, Human, Mouse, Sheep

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human PAFAH1B2 (101-200/229aa)
Target	PAFAH1B2
Molecular Weight	25 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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PA1B2_HUMAN; PAF acetylhydrolase 30 kDa subunit; PAF AH 30 kDa subunit; PAF AH beta subunit; PAF AH subunit beta; PAF-AH 30 kDa subunit; PAF-AH subunit beta; PAFAH 30 kDa subunit; PAFAH beta subunit; PAFAH subunit beta; Pafah1b2; PAFAH1B2 protein; PAFAHB; Platelet activating factor acetylhydrolase IB beta subunit; Platelet activating factor acetylhydrolase IB subunit beta; Platelet activating factor acetylhydrolase isoform Ib beta subunit; Platelet-activating factor acetylhydrolase IB subunit beta.

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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PAFAH1B2) Polyclonal Antibody, Unconjugated (orb1109) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

PAFAH1B2

UniProt

P68402

UniProt Details

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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WB (1)

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Human (1)

Qianping Zhang # 1, Tiantian Sun # 1, Fan Yu 1, Wei Liu 1, Jin Gao 1, Jinyu Chen 2, Hao Zheng 1, Jinming Liu 1, Chenjian Miao 3, Huanyi Guo 3, Wu Tian 4, Meihui Su 5, Yingjie Guo 6, Xi Liu 1, Yandong Pei 1, Zhuofei Wang 7, Shang Chen 8, Chenglong Mu 1, Sin Man Lam 9 10, Guanghou Shui 9 11, Zongjin Li 8, Zhongbo Yu 7, Yan Zhang 4, Guo Chen 1, Congcong Lu 1, Adam C Midgley 12, Changhua Li 5, Xin Bian 1, Xudong Liao 1, Yong Wang 13, Wei Xiong 14, Hongying Zhu 15, Yanjun Li 16, Quan Chen PAFAH2 suppresses synchronized ferroptosis to ameliorate acute kidney injury Nat Chem Biol ., (2024)

PubMed: 38287154

Applications

Reactivity

Human