PAFR/PAF Receptor Rabbit Polyclonal Antibody

SKU: orb11225

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Description

Research Area

Cardiovascular Research, G Protein-Coupled Receptors, GPCR Signaling Pathway, Inflammation, Macrophage

Images & Validation

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Item 1 of 3

Tested Applications	FC, IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Bovine, Canine, Gallus, Rabbit

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human PAFR (231-342/342aa)
Target	PTAFR
Molecular Weight	38 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Platelet-activating factor receptor; PAF receptor; Ptafr; OTTHUMP00000004041; OTTMUSP00000010350; PAF R; PAF-R; PAFR; Platelet activating factor receptor; Platelet-activating factor receptor; PTAFR; PTAFR_HUMAN; RP23-470B20.1.

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PAFR/PAF Receptor Rabbit Polyclonal Antibody

Blank control: 293T cells (blue). Primary Antibody: Rabbit Anti-PAFR/PAF antibody (orb11225), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (orb11225, 1 µg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.

Sample: Lane 1: Lung (Rat) Lysate at 30 ug, Lane 2: Brain (Rat) Lysate at 30 ug, Primary: Anti-PTAFR (orb11225) at 1:200 dilution, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:3000 dilution, Predicted band size: 38kD, Observed band size: 38kD.

Sample: Lymph (Mouse) Lysate at 30 ug, Primary: Anti-RAFR/PAF (orb11225) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution, Predicted band size: 38 kD, Observed band size: 38 kD.

Quick Database Links

Gene Symbol

PTAFR

UniProt

P25105

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

Flow Cytometry

Immunofluorescence