Phospho-Alpha synuclein (Ser129) Rabbit Polyclonal Antibody

SKU: orb7038

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Description

Research Area

Neuroscience, Synuclein

Images & Validation

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Item 1 of 4

Tested Applications	FC, WB
Dilution range	WB=1:500-2000, Flow-Cyt=1ug/Test
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Equine, Porcine, Rabbit, Sheep

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated Synthesised phosphopeptide derived from human SNCA around the phosphorylation site of Ser129 MP(p-S)EE
Target	SNCA
Molecular Weight	15 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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SNCA(phospho S129); alpha Synuclein(phospho S129); alpha-Synuclein(phospho-Ser129); Synuclein alpha(phospho-Ser129); SNCA; SYUA_HUMAN; alpha synuclein; alpha-synuclein, isoform NACP140; alpha SYN; MGC105443; MGC110988; MGC127560; MGC64356; NACP; Non A beta component of AD amyloid; Non A4 component of amyloid precursor; Non-A-beta component of alzheimers disease amyloid, precursor of; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; PARK 1; PARK 4; PARK1; PARK4; Parkinson disease(autosomal dominant, Lewy body) 4; Parkinson disease familial 1; Syn; Snca synuclein, alpha(non A4 component of amyloid precursor); Synuclein alpha; Synuclein, alpha(non A4 component of amyloid precursor); Synuclein-α; Synuclein α.

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Phospho-Alpha synuclein (Ser129) Rabbit Polyclonal Antibody

Blank control (Black line): HUVEC (Black). Primary Antibody (green line): Rabbit Anti-phospho-Alpha synuclein (Ser129) antibody (orb7038), dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Cerebellum (Mouse) Lysate at 40 ug, Lane 2: Cerebrum (Mouse) Lysate at 40 ug, Lane 3: Cerebellum (Rat) Lysate at 40 ug, Lane 4: Cerebrum (Rat) Lysate at 40 ug, Lane 5: SH-SY5Y (Human) Cell Lysate at 30 ug, Lane 6: U251 (Human) Cell Lysate at 30 ug, Lane 7: 293T (Human) Cell Lysate at 30 ug, Lane 8: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-Alpha synuclein (Ser129) (orb7038) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 18 kD, Observed band size: 18 kD.

Sample: Lane 1: Human SY5Y cell lysates, Lane 2: Human U87MG cell lysates, Lane 3: Human U251 cell lysates, Lane 4: Human HUVEC Cell Lysates, Lane 5: Human HELA Cell Lysates, Lane 6: Human 293T Cell Lysates, Primary: Anti-phospho-Alpha synuclein (Ser129) (orb7038) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 15 kDa, Observed band size: 18 kDa.

Sample: RAW264.7 (Mouse) Cell Lysate at 40 ug, Primary: Anti-phospho-Alpha synuclein (Ser129) (orb7038) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 15 kD, Observed band size: 15 kD.

Quick Database Links

Gene Symbol

SNCA

UniProt

P37840

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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