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Phospho-AQP2 (Ser269) Rabbit Polyclonal Antibody

SKU: orb4533

Description

Phospho-AQP2 (Ser269) Rabbit Polyclonal Antibody

Research Area

Signal Transduction

Images & Validation

Tested ApplicationsIF, IHC-Fr, IHC-P
Dilution RangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
ReactivityRat
Predicted ReactivityBovine, Canine, Mouse, Porcine

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated Synthesised phosphopeptide derived from mouse AQP2 around the phosphorylation site of Ser269 RG(p-S)KA
TargetAQP2
Molecular Weight30 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

AQP2 (p-S269); p-AQP2; phospho-AQP2; AQP-CD; NDI2; WCH-CD; cph; jpk; AQP-2; aquaporin-2; AQP2_HUMAN; AQP2; ADH water channel; Aquaporin-CD (AQP-CD); Collecting duct water channel protein; Water channel protein for renal collecting duct; AQP2_MOUSE; AQP2_RAT;

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Phospho-AQP2 (Ser269) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat kidney tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (AQP2 (Ser269)) Polyclonal Antibody, Unconjugated (orb4533) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

UniProt Details

No UniProt data available

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Protocol Information

IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
IF
Immunofluorescence
View Protocol

Phospho-AQP2 (Ser269) Rabbit Polyclonal Antibody (orb4533)

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Available Sizes

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50 μl
¥ 3,250.00
100 μl
¥ 5,070.00
200 μl
¥ 6,110.00
DispatchUsually dispatched within 5-10 working days
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